Kuo_2012_Bioprocess.Biosyst.Eng_35_1137

Reference

Title : Lipase catalyzed acetylation of 3,5,4'-trihydroxystilbene: optimization and kinetics study - Kuo_2012_Bioprocess.Biosyst.Eng_35_1137
Author(s) : Kuo CH , Hsiao FW , Dai SM , Chang CM , Lee CC , Liu YC , Shieh CJ
Ref : Bioprocess Biosyst Eng , 35 :1137 , 2012
Abstract :

The use of immobilized lipase from Candida antarctica Novozym((R 435 to catalyze acetylation of trans-3,5,4'-trihydroxystilbene was investigated in this study Response surface methodology and 5-level-4-factor central composite rotatable design were adopted to evaluate the effects of synthesis variables including reaction time 24-72 h temperature 25-65 degrees C substrate molar ratio 1:15-1:75 and enzyme amount 600-3,000 PLU on the percentage molar conversion of trans-4'-O-acetyl-3,5-dihydroxystilbene The results showed that reaction temperature and enzyme amount were the most important parameters on percentage molar conversion Based on ridge max analysis the optimum conditions for synthesis were reaction time 60 h reaction temperature 64 degrees C substrate molar ratio 1:56 and enzyme amount 2,293 PLU The molar conversion of actual experimental values was 95 under optimal conditions The synthesis product was analyzed using HPLC mass and NMR The results revealed that the major product was trans-4'-O-acetyl-3,5-dihydroxystilbene The reaction kinetics was found to follow the Ping-Pong mechanism substrate inhibition was not found at high vinyl acetate concentration.

PubMedSearch : Kuo_2012_Bioprocess.Biosyst.Eng_35_1137
PubMedID: 22349988

Related information

Citations formats

Kuo CH, Hsiao FW, Dai SM, Chang CM, Lee CC, Liu YC, Shieh CJ (2012)
Lipase catalyzed acetylation of 3,5,4'-trihydroxystilbene: optimization and kinetics study
Bioprocess Biosyst Eng 35 :1137

Kuo CH, Hsiao FW, Dai SM, Chang CM, Lee CC, Liu YC, Shieh CJ (2012)
Bioprocess Biosyst Eng 35 :1137