Kwon_2012_Pestic.Biochem.Physiol_103_94

Reference

Title : Cloning of the acetylcholinesterase 1 gene and identification of point mutations putatively associated with carbofuran resistance in Nilaparvata lugens - Kwon_2012_Pestic.Biochem.Physiol_103_94
Author(s) : Kwon DH , Cha DJ , Kim YH , Lee SW , Lee SH
Ref : Pesticide Biochemistry and Physiology , 103 :94 , 2012
Abstract :

Molecular mechanisms of carbofuran resistance in the brown planthopper, Nilaparvata lugens Stl, were investigated. A carbofuran-resistant strain (CAS) showed approximately 45.5- and 15.1-fold resistance compared with a susceptible strain (SUS) and a non-selected field strain (FM), respectively. Activities of the esterase and mixed-function oxidase were approximately 2.8- and 1.6-fold higher, respectively, in the CAS strain than in the SUS strain, suggesting that these enzymes play a minor role in carbofuran resistance. Interestingly, the insensitivity of acetylcholinesterase (AChE) to carbofuran was approximately 5.5- and 3.7-fold higher in the CAS strain compared to the SUS and FM strains, respectively, indicating that AChE insensitivity is associated with carbofuran resistance. Western blot analysis identified two kinds of AChEs, of which the type-1 AChE (encoded from Nlace1, which is paralogous to the Drosophila AChE gene) was determined to be the major catalytic AChE in N. lugens. The open reading frame of Nlace1 is composed of 1989 bp (approximately 74 kD) and revealed 52.5% and 24.3% amino acid sequence identities to those of Nephotettix cincticeps and Drosophila melanogaster, respectively. Screening of point mutations identified four amino acid substitutions (G119A, F/Y330S, F331H and H332L) in the CAS strain that likely contribute to AChE insensitivity. The frequencies of these mutations were well correlated with resistance levels, confirming that they are associated with reduced sensitivity to carbofuran in N. lugens. These point mutations can be useful as genetic markers for monitoring resistance levels in field populations of N. lugens.

PubMedSearch : Kwon_2012_Pestic.Biochem.Physiol_103_94
PubMedID:
Gene_locus related to this paper: nillu-ACHE1

Citations formats

Kwon DH, Cha DJ, Kim YH, Lee SW, Lee SH (2012)
Cloning of the acetylcholinesterase 1 gene and identification of point mutations putatively associated with carbofuran resistance in Nilaparvata lugens
Pesticide Biochemistry and Physiology 103 :94

Kwon DH, Cha DJ, Kim YH, Lee SW, Lee SH (2012)
Pesticide Biochemistry and Physiology 103 :94