LaRochelle_1989_Dev.Biol_132_130

Genetic variants of the C2 muscle cell line were used to investigate the relation between acetylcholine receptor (AChR) clustering and clustering of the 43-kDa protein. Two variants that express severely reduced amounts of the alpha subunit of the AChR and consequently lack AChR clusters were found also to lack clusters of the 43-kDa protein. The amount of 43-kDa protein in the variants measured by immunoassay was reduced to about one-third the levels found in wild-type cells. The beta subunit of the AChR was reduced to a similar extent. Northern blot analysis showed that neither the 43-kDa protein mRNA nor the beta subunit mRNA was reduced in the variants. Taken together, these results suggest that the amounts of beta subunit and 43-kDa protein may be regulated coordinately by a post-transcriptional mechanism.