| Title : Genomically recoded organisms expand biological functions - Lajoie_2013_Science_342_357 |
| Author(s) : Lajoie MJ , Rovner AJ , Goodman DB , Aerni HR , Haimovich AD , Kuznetsov G , Mercer JA , Wang HH , Carr PA , Mosberg JA , Rohland N , Schultz PG , Jacobson JM , Rinehart J , Church GM , Isaacs FJ |
| Ref : Science , 342 :357 , 2013 |
|
Abstract :
We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance. |
| PubMedSearch : Lajoie_2013_Science_342_357 |
| PubMedID: 24136966 |
| Gene_locus related to this paper: ecoli-YfhR |
| Gene_locus | ecoli-YfhR |
Lajoie MJ, Rovner AJ, Goodman DB, Aerni HR, Haimovich AD, Kuznetsov G, Mercer JA, Wang HH, Carr PA, Mosberg JA, Rohland N, Schultz PG, Jacobson JM, Rinehart J, Church GM, Isaacs FJ (2013)
Genomically recoded organisms expand biological functions
Science
342 :357
Lajoie MJ, Rovner AJ, Goodman DB, Aerni HR, Haimovich AD, Kuznetsov G, Mercer JA, Wang HH, Carr PA, Mosberg JA, Rohland N, Schultz PG, Jacobson JM, Rinehart J, Church GM, Isaacs FJ (2013)
Science
342 :357