Laney_1997_Biophys.J_72_806

Reference

Title : Changes in the elastic properties of cholinergic synaptic vesicles as measured by atomic force microscopy - Laney_1997_Biophys.J_72_806
Author(s) : Laney DE , Garcia RA , Parsons SM , Hansma HG
Ref : Biophysical Journal , 72 :806 , 1997
Abstract :

Cholinergic synaptic vesicles from Torpedo californica have been probed with the atomic force microscope in aqueous buffers to map and measure their elastic properties. Elastic properties were mapped with a new atomic force microscope technique known as force mapping. Force mapping of vesicles showed that the centers of the vesicles are harder or stiffer than the peripheral areas in the three buffers that were investigated. These were an isoosmotic buffer, a hypoosmotic buffer, and an isoosmotic buffer with 5 mM CaCl2 added. The hardness of the vesicular centers was quantified by calculation of the elastic modulus. Elastic moduli were in the range of 2-13 x 10(5) Pa. Vesicular centers were hardest in calcium-containing buffer and softest in isoosmotic buffer. Hypotheses are presented for the composition and function of the hard centers.

PubMedSearch : Laney_1997_Biophys.J_72_806
PubMedID: 9017205

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Citations formats

Laney DE, Garcia RA, Parsons SM, Hansma HG (1997)
Changes in the elastic properties of cholinergic synaptic vesicles as measured by atomic force microscopy
Biophysical Journal 72 :806

Laney DE, Garcia RA, Parsons SM, Hansma HG (1997)
Biophysical Journal 72 :806