Title : Esterase isoenzymes and insecticide resistance in Frankliniella occidentalis populations from the south-east region of Spain - Lapez-Soler_2008_Pest.Manag.Sci_64_1258 |
Author(s) : Lapez-Soler N , Cervera A , Moores GD , Martanez-Pardo R , Garcera MD |
Ref : Pest Manag Sci , 64 :1258 , 2008 |
Abstract :
BACKGROUND: Frankliniella occidentalis (Pergande) is among the most important crop pests in the south-east region of Spain; its increasing resistance to insecticides constitutes a serious problem, and understanding the mechanisms involved is therefore of great interest. To this end, F. occidentalis populations, collected from the field at different locations in south-east Spain, were studied in terms of total esterase activity and esterase isoenzyme pattern. RESULTS: Individual thrips extracts were analysed by native polyacrylamide gel electrophoresis (PAGE) and stained for esterase activity with the model substrate alpha-naphthyl acetate. Significant correlations were found between resistance to the insecticides acrinathrin and methiocarb and the presence of a group of three intensely stained bands, named Triplet A. For each individual thrips extract, total esterase activity towards the substrates alpha-naphthyl acetate and alpha-naphthyl butyrate was also measured in a microplate reader. Insects possessing Triplet A showed a significantly higher alpha-naphthyl acetate specific activity and alpha-naphthyl acetate/alpha-naphthyl butyrate activity ratio. This observation allowed a reliable classification of susceptible or resistant insects either by PAGE analysis or by total esterase activity determination. CONCLUSION: The PAGE and microplate assays described can be used as a monitoring technique for detecting acrinathrin- and methiocarb-resistant individuals among F. occidentalis field populations. |
PubMedSearch : Lapez-Soler_2008_Pest.Manag.Sci_64_1258 |
PubMedID: 18688781 |
Lapez-Soler N, Cervera A, Moores GD, Martanez-Pardo R, Garcera MD (2008)
Esterase isoenzymes and insecticide resistance in Frankliniella occidentalis populations from the south-east region of Spain
Pest Manag Sci
64 :1258
Lapez-Soler N, Cervera A, Moores GD, Martanez-Pardo R, Garcera MD (2008)
Pest Manag Sci
64 :1258