| Title : Identification of a serotonin receptor coupled to adenylyl cyclase involved in learning-related heterosynaptic facilitation in Aplysia - Lee_2009_Proc.Natl.Acad.Sci.U.S.A_106_14634 |
| Author(s) : Lee YS , Choi SL , Lee SH , Kim H , Park H , Lee N , Chae YS , Jang DJ , Kandel ER , Kaang BK |
| Ref : Proc Natl Acad Sci U S A , 106 :14634 , 2009 |
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Abstract :
Serotonin (5-HT) plays a critical role in modulating synaptic plasticity in the marine mollusc Aplysia and in the mammalian nervous system. In Aplysia sensory neurons, 5-HT can activate several signal cascades, including PKA and PKC, presumably via distinct types of G protein-coupled receptors. However, the molecular identities of these receptors have not yet been identified. We here report the cloning and functional characterization of a 5-HT receptor that is positively coupled to adenylyl cyclase in Aplysia neurons. The cloned receptor, 5-HT(apAC1), stimulates the production of cAMP in HEK293T cells and in Xenopus oocytes. Moreover, the knockdown of 5-HT(apAC1) expression by RNA interference blocked 5-HT-induced cAMP production in Aplysia sensory neurons and blocked synaptic facilitation in nondepressed or partially depressed sensory-to-motor neuron synapses. These data implicate 5-HT(apAC1) as a major modulator of learning related synaptic facilitation in the direct sensory to motor neuron pathway of the gill withdrawal reflex. |
| PubMedSearch : Lee_2009_Proc.Natl.Acad.Sci.U.S.A_106_14634 |
| PubMedID: 19706550 |
Lee YS, Choi SL, Lee SH, Kim H, Park H, Lee N, Chae YS, Jang DJ, Kandel ER, Kaang BK (2009)
Identification of a serotonin receptor coupled to adenylyl cyclase involved in learning-related heterosynaptic facilitation in Aplysia
Proc Natl Acad Sci U S A
106 :14634
Lee YS, Choi SL, Lee SH, Kim H, Park H, Lee N, Chae YS, Jang DJ, Kandel ER, Kaang BK (2009)
Proc Natl Acad Sci U S A
106 :14634