Lee_2014_Protein.Expr.Purif_101C_91

Reference

Title : A stress-responsive Escherichia coli protein, CysQ is a highly effective solubility enhancer for aggregation-prone heterologous proteins - Lee_2014_Protein.Expr.Purif_101C_91
Author(s) : Lee JH , Lee JY , Song JA , Han KY , Lee DS , Lee J
Ref : Protein Expr Purif , 101C :91 , 2014
Abstract :

When used as an N-terminal fusion expression partner, the Escherichia coli stress-responsive protein, CysQ dramatically increased the cytoplasmic solubility of various aggregation-prone heterologous proteins: Pseudomonas putida cutinase (CUT), human granulocyte colony-stimulating factor (hG-CSF), human ferritin light chain (hFTN-L), arginine deiminase (ADI), human interleukin-2 (IL2), human activation induced cytidine deaminase (AID), and deletion mutant of human glutamate decarboxylase (GAD448-585). As compared with well-known fusion tags such as glutathione-S-transferase (GST) and maltose-binding protein (MBP), the performance of CysQ as solubility enhancer was evidently better than GST and was similar to or better than MBP for the seven heterologous proteins above. This is likely due to the intrinsic ability of CysQ to form its native conformation, probably promoting the binding of molecular chaperones during the folding of CysQ-fusion protein. When used as a substrate, p-nitrophenyl butyrate (PNB) was successfully hydrolyzed to p-nitrophenol by CysQ-CUT fusion mutant. Even after CysQ was removed, the solubility of hFTN-L and hG-CSF, the secondary structure of hG-CSF, and self-assembly activity of hFTN-L were successfully maintained. Conclusively, it seems that CysQ is a highly effective solubility enhancer and fusion expression partner for the production of a variety of bio-active recombinant proteins.

PubMedSearch : Lee_2014_Protein.Expr.Purif_101C_91
PubMedID: 24945073

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Citations formats

Lee JH, Lee JY, Song JA, Han KY, Lee DS, Lee J (2014)
A stress-responsive Escherichia coli protein, CysQ is a highly effective solubility enhancer for aggregation-prone heterologous proteins
Protein Expr Purif 101C :91

Lee JH, Lee JY, Song JA, Han KY, Lee DS, Lee J (2014)
Protein Expr Purif 101C :91