Li_2003_J.Biol.Chem_278_2799

Reference

Title : Receptor-ligand interaction between vitellogenin receptor (VtgR) and vitellogenin (Vtg), implications on low density lipoprotein receptor and apolipoprotein B\/E. The first three ligand-binding repeats of VtgR interact with the amino-terminal region of Vtg - Li_2003_J.Biol.Chem_278_2799
Author(s) : Li A , Sadasivam M , Ding JL
Ref : Journal of Biological Chemistry , 278 :2799 , 2003
Abstract :

The vitellogenin receptor (VtgR) belongs to the low density lipoprotein receptor (LDLR) gene family. It mediates the uptake of vitellogenin (Vtg) in oocyte development of oviparous animals. In this study, we cloned and characterized two forms of Oreochromis aureus VtgR. Northern analysis showed that VtgR was specifically expressed in ovarian tissues. However, reverse transcription-PCR indicates that either there are trace levels of expression of VtgR or a homolog of LDLR exists in nonovarian tissues. The VtgR is highly homologous to the very low density lipoprotein receptor. To better understand the mechanism by which similar structural modules in the ligand-binding domain bind different ligands, we used the yeast two-hybrid system to screen for the minimal interaction motifs in Vtg and VtgR. The amino-terminal region of the lipovitellin I domain of Vtg interacts with the ligand-binding domain of VtgR. The first three ligand-binding repeats of the receptor were found to be essential for ligand binding. Computational analysis of the binding sequence indicates that Vtg has a similar receptor-binding region to apolipoprotein (apo) E and apoB. Site-directed mutagenesis of this region indicates electrostatic interaction between Vtg and its receptor. Sequence analysis suggests the coevolution of receptor-ligand pairs for the LDLR/apo superfamily and suggests that the mode of binding of LDLR/very low density lipoprotein receptor to apoB and apoE is inherited from the electrostatic attraction of VtgR and Vtg.

PubMedSearch : Li_2003_J.Biol.Chem_278_2799
PubMedID: 12429745

Related information

Citations formats

Li A, Sadasivam M, Ding JL (2003)
Receptor-ligand interaction between vitellogenin receptor (VtgR) and vitellogenin (Vtg), implications on low density lipoprotein receptor and apolipoprotein B\/E. The first three ligand-binding repeats of VtgR interact with the amino-terminal region of Vtg
Journal of Biological Chemistry 278 :2799

Li A, Sadasivam M, Ding JL (2003)
Journal of Biological Chemistry 278 :2799