| Title : Unexpected reversal of the regioselectivity in Thermomyces lanuginosus lipase-catalyzed acylation of floxuridine - Li_2009_Biotechnol.Lett_31_1241 |
| Author(s) : Li N , Zong MH , Ma D |
| Ref : Biotechnol Lett , 31 :1241 , 2009 |
|
Abstract :
Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. The possible reason might be the presence of a remote interaction between the unsaturated bond in the acyl group and the aromatic ring of amino acid residue Trp89 in the lid of the lipase. |
| PubMedSearch : Li_2009_Biotechnol.Lett_31_1241 |
| PubMedID: 19360390 |
Li N, Zong MH, Ma D (2009)
Unexpected reversal of the regioselectivity in Thermomyces lanuginosus lipase-catalyzed acylation of floxuridine
Biotechnol Lett
31 :1241
Li N, Zong MH, Ma D (2009)
Biotechnol Lett
31 :1241