Title : Regulation of gene expression by FSP27 in white and brown adipose tissue - Li_2010_BMC.Genomics_11_446 |
Author(s) : Li D , Zhang Y , Xu L , Zhou L , Wang Y , Xue B , Wen Z , Li P , Sang J |
Ref : BMC Genomics , 11 :446 , 2010 |
Abstract :
BACKGROUND: Brown and white adipose tissues (BAT and WAT) play critical roles in controlling energy homeostasis and in the development of obesity and diabetes. The mouse Fat-Specific protein 27 (FSP27), a member of the cell death-inducing DFF45-like effector (CIDE) family, is expressed in both BAT and WAT and is associated with lipid droplets. Over-expression of FSP27 promotes lipid storage, whereas FSP27 deficient mice have improved insulin sensitivity and are resistant to diet-induced obesity. In addition, FSP27-deficient white adipocytes have reduced lipid storage, smaller lipid droplets, increased mitochondrial activity and a higher expression of several BAT-selective genes. To elucidate the molecular mechanism by which FSP27 controls lipid storage and gene expression in WAT and BAT, we systematically analyzed the gene expression profile of FSP27-deficient WAT by microarray analysis and compared the expression levels of a specific set of genes in WAT and BAT by semi-quantitative real-time PCR analysis. |
PubMedSearch : Li_2010_BMC.Genomics_11_446 |
PubMedID: 20649970 |
Li D, Zhang Y, Xu L, Zhou L, Wang Y, Xue B, Wen Z, Li P, Sang J (2010)
Regulation of gene expression by FSP27 in white and brown adipose tissue
BMC Genomics
11 :446
Li D, Zhang Y, Xu L, Zhou L, Wang Y, Xue B, Wen Z, Li P, Sang J (2010)
BMC Genomics
11 :446