Title : Biocatalytic synthesis of L-ascorbyl palmitate using oleic acid imprinted Aspergillus niger lipase immobilized on resin - Li_2024_Food.Chem_463_141075 |
Author(s) : Li Q , Qian J , Huang A , Shi B , Gu G |
Ref : Food Chem , 463 :141075 , 2024 |
Abstract :
In order to improve the esterification efficiency of the enzymatic synthesis of l-ascorbic acid palmitate, the substrate analogue imprinting of the Aspergillus niger lipase-catalyzed esterification process was studied. Oleic acid was selected as the imprinting molecule, oleic acid imprinting immobilized lipase was prepared at pH 8.0, 0.1 g oleic acid, 1.5 mL of 95 % ethanol, and 0.1 g Tween-20. Through solubilization and supersaturation of Vitamin C, the reaction concentration of Vitamin C reached 5.00 % (m/v) in dioxane with 93.99 % esterification rate and 110.72 g/L of product concentration. Moreover, the Vitamin C reaction concentration can reach 8.00 % by using staged substrate feeding, and the esterification rate and product concentration of esterification after 28 h was 156.34 g/L and 82.96 %. Besides, the imprinting-induced conformational changes in enzyme proteins was characterized by fluorescence and infrared spectroscopy. This method provides a pathway for enzymatic production of l-ascorbic acid palmitate. |
PubMedSearch : Li_2024_Food.Chem_463_141075 |
PubMedID: 39241420 |
Li Q, Qian J, Huang A, Shi B, Gu G (2024)
Biocatalytic synthesis of L-ascorbyl palmitate using oleic acid imprinted Aspergillus niger lipase immobilized on resin
Food Chem
463 :141075
Li Q, Qian J, Huang A, Shi B, Gu G (2024)
Food Chem
463 :141075