Li_2024_Food.Chem_464_141625

In this paper, a camphor-derived enzyme activatable probe CPA was synthesized for detecting dimethoate pesticide. The ester bond of probe CPA could be selectively hydrolyzed and fragmented in the presence of carboxylesterase (CE), which caused a remarkably enhanced green fluorescence signal at 501 nm. Probe CPA could function as an effective fluorescent platform for the sequential detection of dimethoate due to the obvious inhibition effect of dimethoate on the activity of CE. The detection limit of probe CPA to dimethoate was computed to be 0.1104 microg/mL. Even more important, CPA was effectively utilized for quantitative determination of trace dimethoate residues in agricultural products including fresh vegetables and fruits with good accuracy. Furthermore, the probe CPA could realize the detection of dimethoate in living cells and zebrafish. This work is expected to provide a highly sensitive and accurate analytical method for detecting organophosphorus pesticide residues in food samples and biological systems.