Li_2025_Naunyn.Schmiedebergs.Arch.Pharmacol__

This study aims to investigate the material basis and underlying mechanisms of action of the Dan Zhi Qing'e Formula in the treatment of menopausal hot flashes. The composition analysis of the Dan Zhi Qing'e Formula was conducted using UHPLC-Q-TOF MS, facilitated by MassLynx V4.1 software. Subsequently, Cytoscape 3.10.1 was employed to merge the data with information from Swiss, GeneCards, and OMIM databases to identify the active components and primary targets. GO and KEGG analyses were performed to elucidate the potential mechanisms of action. Finally, the molecular docking technique was applied to validate the results. A total of 118 components, 73 blood-absorbed components, and 89 potential targets were identified. Seven key targets were obtained, which were aldose reductase (AKR1B1, UniProtKB: P15121), carbonic anhydrase 4 (CA4, UniProtKB: P22748), carbonic anhydrase 2 (CA2, UniProtKB: P00918), acetylcholinesterase (ACHE, UniProtKB: P22303), estrogen receptor beta (ESR2, UniProtKB: Q92731), cytochrome P450 19A1 (CYP19A1, UniProtKB: P11511), and matrix metalloproteinase-2 (MMP2, UniProtKB: P08253). Molecular docking studies indicate that these core components exhibit strong affinity for the identified targets. These targets contribute to the tonic function of the liver and kidneys through hormone response. The findings provide a scientific foundation for further in-depth research into the therapeutic mechanisms of Dan Zhi Qing'e Formula for menopausal hot flashes.