Title : Immobilization of Pseudomonas fluorescens lipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems - Lima_2015_J.Ind.Microbiol.Biotechnol_42_523 |
Author(s) : Lima LN , Oliveira GC , Rojas MJ , Castro HF , Da Ros PC , Mendes AA , Giordano RL , Tardioli PW |
Ref : J Ind Microbiol Biotechnol , 42 :523 , 2015 |
Abstract :
This work describes the preparation of biocatalysts for ethanolysis of soybean and babassu oils in solvent-free systems. Polystyrene, Amberlite (XAD-7HP), and octyl-silica were tested as supports for the immobilization of Pseudomonas fluorescens lipase (PFL). The use of octyl-silica resulted in a biocatalyst with high values of hydrolytic activity (650.0 +/- 15.5 IU/g), immobilization yield (91.3 +/- 0.3 %), and recovered activity (82.1 +/- 1.5 %). PFL immobilized on octyl-silica was around 12-fold more stable than soluble PFL, at 45 degrees C and pH 8.0, in the presence of ethanol at 36 % (v/v). The biocatalyst provided high vegetable oil transesterification yields of around 97.5 % after 24 h of reaction using babassu oil and around 80 % after 48 h of reaction using soybean oil. The PFL-octyl-silica biocatalyst retained around 90 % of its initial activity after five cycles of transesterification of soybean oil. Octyl-silica is a promising support that can be used to immobilize PFL for subsequent application in biodiesel synthesis. |
PubMedSearch : Lima_2015_J.Ind.Microbiol.Biotechnol_42_523 |
PubMedID: 25626526 |
Lima LN, Oliveira GC, Rojas MJ, Castro HF, Da Ros PC, Mendes AA, Giordano RL, Tardioli PW (2015)
Immobilization of Pseudomonas fluorescens lipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems
J Ind Microbiol Biotechnol
42 :523
Lima LN, Oliveira GC, Rojas MJ, Castro HF, Da Ros PC, Mendes AA, Giordano RL, Tardioli PW (2015)
J Ind Microbiol Biotechnol
42 :523