Lin_2008_J.Bacteriol_190_515

Reference

Title : Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess - Lin_2008_J.Bacteriol_190_515
Author(s) : Lin TL , Lee CZ , Hsieh PF , Tsai SF , Wang JT
Ref : Journal of Bacteriology , 190 :515 , 2008
Abstract :

Genomic heterogeneity has been shown to be associated with Klebsiella pneumoniae strains causing pyogenic liver abscesses (PLA) and metastatic infections. In order to explore the mechanism responsible for genomic heterogeneity in K. pneumoniae, we compared the complete genomic sequences of strains NTUH-K2044 and MGH78578. An approximately 76-kbp DNA fragment located adjacent to an asparagine (asn) tRNA gene was present in NTUH-K2044 but not in MGH78578. This fragment could be divided into three regions with different functions, and structurally it resembled a functional integrative and conjugative element (ICE), ICEEc1, in Escherichia coli. The 5' region of this fragment contained genes similar to a high-pathogenicity island (HPI) of Yersinia pestis and Yersinia pseudotuberculosis. The middle region was similar to part of a large plasmid in K. pneumoniae, and the 3' region contained genes responsible for DNA conjugative transfer. Therefore, this DNA fragment was designated ICEKp1. Precise excision and extrachromosomal circularization of ICEKp1 were detected in K. pneumoniae wild-type strain NTUH-K2044. ICEKp1 could integrate into the asn tRNA loci of the chromosome of another K. pneumoniae isolate. The prevalence of ICEKp1 was higher in PLA strains (38 of 42 strains) than in non-tissue-invasive strains (5 of 32 strains). Therefore, ICEKp1 may contribute to the transmission of the HPI and result in K. pneumoniae PLA infection-associated genomic heterogeneity.

PubMedSearch : Lin_2008_J.Bacteriol_190_515
PubMedID: 17981959
Gene_locus related to this paper: klepn-q6u5x9

Related information

Gene_locus klepn-q6u5x9

Citations formats

Lin TL, Lee CZ, Hsieh PF, Tsai SF, Wang JT (2008)
Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess
Journal of Bacteriology 190 :515

Lin TL, Lee CZ, Hsieh PF, Tsai SF, Wang JT (2008)
Journal of Bacteriology 190 :515