Title : Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster - Lin_2010_Org.Lett_12_3816 |
Author(s) : Lin S , Horsman GP , Shen B |
Ref : Org Lett , 12 :3816 , 2010 |
Abstract :
Neocarzinostatin (1) biosynthesis is proposed to involve a vicinal diol intermediate. It is reported that NcsF2, one of two epoxide hydrolases encoded by the NCS gene cluster, catalyzes regiospecific addition of H(2)O to C-2 of both (R)- and (S)-styrene oxides to afford (R)- and (S)-1-phenyl-1,2-ethanediols, respectively, supporting its proposed role in 1 biosynthesis. (R)-1-Phenyl-1,2-ethanediol (87% yield and 99% ee) was obtained from (+/-)-styrene oxide hydrolysis by cocatalysis using NcsF2 and SgcF, the complementary epoxide hydrolase from the C-1027 biosynthetic pathway. |
PubMedSearch : Lin_2010_Org.Lett_12_3816 |
PubMedID: 20704329 |
Gene_locus related to this paper: strcz-Q84HB8 |
Gene_locus | strcz-Q84HB8 |
Lin S, Horsman GP, Shen B (2010)
Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster
Org Lett
12 :3816
Lin S, Horsman GP, Shen B (2010)
Org Lett
12 :3816