Title : Effect of Silent Mutations in Translational Initial Region on the Production of Recombinant Cutinase in Escherichia coli - Liu_2011_Curr.Microbiol_62_1302 |
Author(s) : Liu ZG , Zhu L , Zhu KL , Chen S , Chen J , Wu J |
Ref : Curr Microbiol , 62 :1302 , 2011 |
Abstract :
Translational Initial Region (TIR) is the threshold of an intracellular translation process, and tiny alterations in this region are reported to intensely influence the downstream expression. Such property provides a potential utilization in extracellular production of recombinant enzyme. As an esterase, cutinase is an essential catalyst in the process of textile scouring, and has a potential application in food and chemical industry. In the present study, a bacterial cutinase (Tfu_0883) from Thermobifida fusca was expressed in Escherichia coli with pelB as its signal peptide using trc as its promoter. A subsequent TIR degeneracy mutagenesis was then carried out in the initial sequence of pelB. A fast screening method for these mutants was developed and a series of strains with different expression strengths were accordingly obtained. Among these mutants, a high cutinase production level of 38.0 U/ml was achieved, which is three times that of the control group. This study explored the potential utilization of TIR degeneracy mutagenesis in the production of industrial enzymes. |
PubMedSearch : Liu_2011_Curr.Microbiol_62_1302 |
PubMedID: 21212953 |
Liu ZG, Zhu L, Zhu KL, Chen S, Chen J, Wu J (2011)
Effect of Silent Mutations in Translational Initial Region on the Production of Recombinant Cutinase in Escherichia coli
Curr Microbiol
62 :1302
Liu ZG, Zhu L, Zhu KL, Chen S, Chen J, Wu J (2011)
Curr Microbiol
62 :1302