| Title : The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas - Liu_2016_Dev.Comp.Immunol_65_139 |
| Author(s) : Liu Z , Zhou Z , Wang L , Dong W , Qiu L , Song L |
| Ref : Dev Comp Immunol , 65 :139 , 2016 |
| Abstract : Muscarinic receptors, which selectively take muscarine as their ligand, are critical for the immunological and physiological processes in animals. In the present study, the open region frame (ORF) of a homologue of muscarinic acetylcholine (ACh) receptor (mAChR) was amplified from oyster Crassostrea gigas (named as CgmAChR-1), whose full length was 1983 bp and the protein it encoded contained 660 amino acids with a seven transmembrane region. Phylogeny analysis suggested that CgmAChR-1 shared homology with M5 muscarinic receptor found in invertebrates including Habropoda laboriosa, Acromyrmex echinatior and Echinococcus granulosus. After cell transfection of CgmAChR-1 into HEK293T cells and ACh incubation, the level of intracellular Ca(2+) and cAMP increased significantly (p < 0.05). Such trend could be reverted with the addition of M3 and M5 muscarinic receptor antagonists DAMP and DAR. The CgmAChR-1 transcripts were ubiquitously detectable in seven different tissues with the maximal expression level in adductor muscle. When the oysters received LPS stimulation, CgmAChR-1 mRNA expression in haemocyte was increased to the highest level (6.05-fold, p < 0.05) at 24 h, while blocking CgmAChR-1 using receptor antagonists before LPS stimulation promoted the expression of oyster TNF, resulting in the increase of haemocyte apoptosis index. These results suggested that CgmAChR-1 was the key molecule in cholinergic neuroendocrine-immune system contributing to the regulation of TNF expression and apoptosis process. |
| ESTHER : Liu_2016_Dev.Comp.Immunol_65_139 |
| PubMedSearch : Liu_2016_Dev.Comp.Immunol_65_139 |
| PubMedID: 27394930 |
Liu Z, Zhou Z, Wang L, Dong W, Qiu L, Song L (2016)
The cholinergic immune regulation mediated by a novel muscarinic acetylcholine receptor through TNF pathway in oyster Crassostrea gigas
Dev Comp Immunol
65 :139
Liu Z, Zhou Z, Wang L, Dong W, Qiu L, Song L (2016)
Dev Comp Immunol
65 :139