Liu_2022_Comb.Chem.High.Throughput.Screen__

Background Phytomedicine are proven to treat various chronic diseases as these compounds are cost effective, render few or nil side effects. The elucidating the ameliorative effect of phytomedicine on cerebral ischemia may be potent alternative therapy. Citronellol, a monoterpene alcohol is one such phytocompound present is essential oils of Cymbopogon nardus, Pelargonium geraniums and has immense pharmacological properties such as antihyperalgesic, anticonvulsant and antinociceptive. Objective In the present work, the anti-ischemic effect of citronellol in both cellular and animal model of stroke was analyzed. Methods Citronellol pretreated SH-SY5Y cells were subjected to oxygen-glucose deprivation and reperfusion. The cells were assessed for cell viability and LDH quantification. Inflammatory cytokines were estimated in the cell lysate of citronellol pretreated OGD-R induced cells. Healthy young SD rats were pretreated with citronellol and induced with MCAO-R. Control group are sham operated rats treated with saline. Group II MCAO/R induced untreated rats. Group III and IV are rats prior treated with 10 mg/kg and 20 mg/kg citronellol respectively for 7 consecutive days and induced with MCAO/R. Brain edema was analysed by quantifying the water content and the percentage of infract was assessed using TTC staining technique. Acetylcholinesterase activity and the neurological scoring were performed to assess the neuroprotective activity of citronellol. Lipid peroxidation and antioxidant levels were quantified to evaluate the antioxidant activity of citronellol. The anti-inflammatory activity of citronellol was assessed by quantifying proinflammatory cytokines using commercially available ELISA kits. Results Citronellol treatment significantly ameliorated the neuronal damage in both cellular and animal stroke model. Prior treatment of citronellol significantly decreased the inflammatory cytokines and increased the antioxidants. Citronellol treatment effectively protected the rats from MCAO/R induced brain edema. Conclusion Our results confirm citronellol is an effective anti-ischemic drug with antioxidant, anti-inflammatory properties.