| Title : Magnetic silica-coated cutinase immobilized via ELPs biomimetic mineralization for efficient nano-PET degradation - Liu_2024_Int.J.Biol.Macromol__135414 |
| Author(s) : Liu G , Yuan H , Chen Y , Mao L , Yang C , Zhang R , Zhang G |
| Ref : Int J Biol Macromol , :135414 , 2024 |
|
Abstract :
The proliferation of nano-plastic particles (NPs) poses severe environmental hazards, urgently requiring effective biodegradation methods. Herein, a novel method was developed for degrading nano-PET (polyethylene terephthalate) using immobilized cutinases. Nano-PET particles were prepared using a straightforward method, and biocompatible elastin-like polypeptide-magnetic nanoparticles (ELPs-MNPs) were obtained as magnetic cores via biomimetic mineralization. Using one-pot synthesis with the cost-effective precursor tetraethoxysilane (TEOS), silica-coated magnetically immobilized ELPs-tagged cutinase (ET-C@SiO(2)@MNPs) were produced. ET-C@SiO(2)@MNPs showed rapid magnetic separation within 30 s, simplifying recovery and reuse. ET-C@SiO(2)@MNPs retained 86 % of their initial activity after 11 cycles and exhibited superior hydrolytic capabilities for nano-PET, producing 0.515 mM TPA after 2 h of hydrolysis, which was 96.6 % that of free enzymes. Leveraging ELPs biomimetic mineralization, this approach offers a sustainable and eco-friendly solution for PET-nanoplastic degradation, highlighting the potential of ET-C@SiO(2)@MNPs in effective nanoplastic waste management and contributing to environmental protection and sustainable development. |
| PubMedSearch : Liu_2024_Int.J.Biol.Macromol__135414 |
| PubMedID: 39245124 |
Liu G, Yuan H, Chen Y, Mao L, Yang C, Zhang R, Zhang G (2024)
Magnetic silica-coated cutinase immobilized via ELPs biomimetic mineralization for efficient nano-PET degradation
Int J Biol Macromol
:135414
Liu G, Yuan H, Chen Y, Mao L, Yang C, Zhang R, Zhang G (2024)
Int J Biol Macromol
:135414