Llopis_2009_Lab.Chip_9_213

Reference

Title : Lab-on-a-chip for ultrasensitive detection of carbofuran by enzymatic inhibition with replacement of enzyme using magnetic beads - Llopis_2009_Lab.Chip_9_213
Author(s) : Llopis X , Pumera M , Alegret S , Merkoci A
Ref : Lab Chip , 9 :213 , 2009
Abstract :

In this paper an ultrasensitive method to determine toxicity due to pesticides in a glass lab-on-a-chip by means of enzymatic inhibition of acetylcholinesterase immobilised on magnetic beads is described. The reproducible insertion of a controlled amount of enzyme-coupled magnetic beads inside the chip channel and their immobilisation in a capture region with the aid of a magnetic field has been optimised. This procedure enables the easy renewal of the biosensing material after each determination in a highly reproducible manner. Several operational parameters such as the working potential for the selective detection of thiocholine (TCh) on a platinum disc electrode, the TCh detection reproducibility and sensitivity, the electroosmotic flow driving voltage and the inhibition time were also evaluated or optimised. The detection of carbofuran (one of the most toxic carbamate pesticides) has been achieved down to the nanomolar level.

PubMedSearch : Llopis_2009_Lab.Chip_9_213
PubMedID: 19107276

Related information

Citations formats

Llopis X, Pumera M, Alegret S, Merkoci A (2009)
Lab-on-a-chip for ultrasensitive detection of carbofuran by enzymatic inhibition with replacement of enzyme using magnetic beads
Lab Chip 9 :213

Llopis X, Pumera M, Alegret S, Merkoci A (2009)
Lab Chip 9 :213