| Title : Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate - Lomolino_2001_Electrophoresis_22_1021 |
| Author(s) : Lomolino G , Lante A , Crapisi A , Spettoli P , Curioni A |
| Ref : Electrophoresis , 22 :1021 , 2001 |
|
Abstract :
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels. |
| PubMedSearch : Lomolino_2001_Electrophoresis_22_1021 |
| PubMedID: 11358123 |
| Substrate | Fluorescein-diacetate |
Lomolino G, Lante A, Crapisi A, Spettoli P, Curioni A (2001)
Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate
Electrophoresis
22 :1021
Lomolino G, Lante A, Crapisi A, Spettoli P, Curioni A (2001)
Electrophoresis
22 :1021