Lopez-Obando_2021_Plant.Cell__

Reference

Title : The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds function via MAX2-dependent and independent pathways - Lopez-Obando_2021_Plant.Cell__
Author(s) : Lopez-Obando M , Guillory A , Boyer FD , Cornu D , Hoffmann B , Le Bris P , Pouvreau JB , Delavault P , Rameau C , de Saint Germain A , Bonhomme S
Ref : Plant Cell , : , 2021
Abstract :

In angiosperms, the alpha/beta hydrolase DWARF14 (D14), along with the F-box protein MORE AXILLARY GROWTH2 (MAX2), perceives strigolactones (SL) to regulate developmental processes. The key SL biosynthetic enzyme CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8) is present in the moss Physcomitrium patens, and PpCCD8-derived compounds regulate moss extension. The PpMAX2 homolog is not involved in the SL response, but 13 PpKAI2LIKE (PpKAI2L) genes homologous to the D14 ancestral paralog KARRIKIN INSENSITIVE2 (KAI2) encode candidate SL receptors. In Arabidopsis thaliana, AtKAI2 perceives karrikins and the elusive endogenous KAI2-Ligand (KL). Here, germination assays of the parasitic plant Phelipanche ramosa suggested that PpCCD8-derived compounds are likely non-canonical SLs. (+)-GR24 SL analog is a good mimic for PpCCD8-derived compounds in P. patens, while the effects of its enantiomer (-)-GR24, a KL mimic in angiosperms, are minimal. Interaction and binding assays of seven PpKAI2L proteins pointed to the stereoselectivity towards (-)-GR24 for a single clade of PpKAI2L (eu-KAI2). Enzyme assays highlighted the peculiar behavior of PpKAI2L-H. Phenotypic characterization of Ppkai2l mutants showed that eu-KAI2 genes are not involved in the perception of PpCCD8-derived compounds but act in a PpMAX2-dependent pathway. By contrast, mutations in PpKAI2L-G, and -J genes abolished the response to the (+)-GR24 enantiomer, suggesting that PpKAI2L-G, and -J proteins are receptors for moss SLs. Published as a preprint Lopez-Obando_2020_Biorxiv__

PubMedSearch : Lopez-Obando_2021_Plant.Cell__
PubMedID: 34459915
Gene_locus related to this paper: phypa-a9s299 , phypa-a9sg07 , phypa-a9skf7 , phypa-a9slp7 , phypa-a9smq5 , phypa-a9ssf6 , phypa-a9st85 , phypa-a9t1j2 , phypa-a9t9d9 , phypa-a0a7i4d2t7 , phypa-a9tc36 , phypa-a9tql4 , phypa-a9tw81

Related information

Substrate GC242
Gene_locus GC242    phypa-a9s299    phypa-a9sg07    phypa-a9skf7    phypa-a9slp7    phypa-a9smq5    phypa-a9ssf6    phypa-a9st85    phypa-a9t1j2    phypa-a9t9d9    phypa-a0a7i4d2t7    phypa-a9tc36    phypa-a9tql4    phypa-a9tw81

Citations formats

Lopez-Obando M, Guillory A, Boyer FD, Cornu D, Hoffmann B, Le Bris P, Pouvreau JB, Delavault P, Rameau C, de Saint Germain A, Bonhomme S (2021)
The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds function via MAX2-dependent and independent pathways
Plant Cell :

Lopez-Obando M, Guillory A, Boyer FD, Cornu D, Hoffmann B, Le Bris P, Pouvreau JB, Delavault P, Rameau C, de Saint Germain A, Bonhomme S (2021)
Plant Cell :