Title : Cloning and analysis of Candida cylindracea lipase sequences - Lotti_1993_Gene_124_45 |
Author(s) : Lotti Marina , Grandori R , Fusetti F , Longhi S , Brocca S , Tramontano A , Alberghina L , Lotti M |
Ref : Gene , 124 :45 , 1993 |
Abstract :
Lipases (Lip) hydrolyze triglycerides into fatty acids and glycerol. Lip produced by the yeast Candida cylindracea are encoded by multiple genomic sequences. We report the molecular cloning and characterization of three genes from this family. They encode putative mature 57-kDa proteins of 534 amino acids (aa). To date, five Lip-encoding genomic sequences from C. cylindracea have been characterized in our laboratory. The five deduced aa sequences share an overall homology of 80%. These sequences have been aligned with each other and with those of homologous enzymes, the Lip from the mould Geotrichum candidum and the acetylcholinesterase from Torpedo californica, whose three-dimensional structures have been solved by X-ray analysis. The C. cylindracea Lip appear to have a structural organization similar to that described for both enzymes. |
PubMedSearch : Lotti_1993_Gene_124_45 |
PubMedID: 8440480 |
Gene_locus related to this paper: canru-1lipa , canru-3lipa , canru-4lipa , canru-5lipa |
Gene_locus | canru-1lipa canru-3lipa canru-4lipa canru-5lipa |
Lotti Marina, Grandori R, Fusetti F, Longhi S, Brocca S, Tramontano A, Alberghina L, Lotti M (1993)
Cloning and analysis of Candida cylindracea lipase sequences
Gene
124 :45
Lotti Marina, Grandori R, Fusetti F, Longhi S, Brocca S, Tramontano A, Alberghina L, Lotti M (1993)
Gene
124 :45