| Title : Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl - Luo_2022_Biosensors.(Basel)_12_ |
| Author(s) : Luo Y , Wu N , Wang L , Song Y , Du Y , Ma G |
| Ref : Biosensors (Basel) , 12 : , 2022 |
| Abstract : A ratiometric electrochemical biosensor based on a covalent organic framework (COF(Thi-TFPB)) loaded with acetylcholinesterase (AChE) was developed. First, an electroactive COF(Thi-TFPB) with a two-dimensional sheet structure, positive charge and a pair of inert redox peaks was synthesized via a dehydration condensation reaction between positively charged thionine (Thi) and 1,3,5-triformylphenylbenzene (TFPB). The immobilization of AChE on the positively charged electrode surface was beneficial for maintaining its bioactivity and achieving the best catalytic effect; therefore, the positively charged COF(Thi-TFPB) was an appropriate support material for AChE. Furthermore, the COF(Thi-TFPB) provided a stable internal reference signal for the constructed AChE inhibition-based electrochemical biosensor to eliminate various effects which were unrelated to the detection of carbaryl. The sensor had a linear range of 2.2-60 microM with a detection limit of 0.22 microM, and exhibited satisfactory reproducibility, stability and anti-interference ability for the detection of carbaryl. This work offers a possibility for the application of COF-based materials in the detection of low-level pesticide residues. |
| ESTHER : Luo_2022_Biosensors.(Basel)_12_ |
| PubMedSearch : Luo_2022_Biosensors.(Basel)_12_ |
| PubMedID: 36005021 |
Luo Y, Wu N, Wang L, Song Y, Du Y, Ma G (2022)
Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl
Biosensors (Basel)
12 :
Luo Y, Wu N, Wang L, Song Y, Du Y, Ma G (2022)
Biosensors (Basel)
12 :