Mahlknecht_2015_Biochem.Biophys.Res.Commun_465_218

Reference

Title : Multimerization of ERBB2\/HER2 specific aptamer leads to improved receptor binding - Mahlknecht_2015_Biochem.Biophys.Res.Commun_465_218
Author(s) : Mahlknecht G , Maron R , Schechter B , Yarden Y , Sela M
Ref : Biochemical & Biophysical Research Communications , 465 :218 , 2015
Abstract :

Aptamers represent a promising new treatment modality for cancer. Specificity and high affinity are two parameters that characterize aptamers. In this work, we elucidated physicochemical parameters of an ERBB2/HER2 specific aptamer and determined an optimal multimerization state, leading to higher binding and improved avidity. We applied biochemical, immunochemical and biophysical methodologies to characterize binding behaviors of multimerized versions of an ERBB2/HER2 specific aptamer and demonstrate structural integrity. Finally, we show that the trimeric ERBB2/HER2 specific aptamer instigates no immunogenic response in vivo. In summary, the set of methodologies we employed establishes a way to enhance activity of a model HER2-aptamer.

PubMedSearch : Mahlknecht_2015_Biochem.Biophys.Res.Commun_465_218
PubMedID: 26248137

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Citations formats

Mahlknecht G, Maron R, Schechter B, Yarden Y, Sela M (2015)
Multimerization of ERBB2\/HER2 specific aptamer leads to improved receptor binding
Biochemical & Biophysical Research Communications 465 :218

Mahlknecht G, Maron R, Schechter B, Yarden Y, Sela M (2015)
Biochemical & Biophysical Research Communications 465 :218