Manjari_2023_IBRO.Neurosci.Rep_15_116

Reference

Title : Unprecedented effect of vitamin D3 on T-cell receptor beta subunit and alpha7 nicotinic acetylcholine receptor expression in a 3-nitropropionic acid induced mouse model of Huntington's disease - Manjari_2023_IBRO.Neurosci.Rep_15_116
Author(s) : Manjari S , Abraham SM , Poornima R , Chaturvedi RK , Maity S , Komal P
Ref : IBRO Neurosci Rep , 15 :116 , 2023
Abstract :

INTRODUCTION: 3-NP induction in rodent models has been shown to induce selective neurodegeneration in the striatum followed by the cortex (Brouillet, 2014). However, it remains unclear whether, under such a neurotoxic condition, characterized by neuroinflammation and oxidative stress, the gene expression of the immune resident protein, T-cell receptor beta subunit (TCR-beta), alpha7 nicotinic acetylcholine receptor (alpha7 nAChRs), the nuclear factor kappa B (NF-kappaB), inflammatory cytokines (TNF-alpha and IL-6), and antioxidants (Cat and GpX4) get modulated on Vitamin D3 (VD) supplementation in the central nervous system. METHODS: In the present study, real-time polymerase chain reaction (RT-PCR) was performed to study the expression of respective genes. Male C57BL/6 mice (8-12 weeks) were divided into four groups namely, Group I: Control (saline); Group II: 3-NP induction via i.p (HD); Group III: Vitamin D3 (VD) and Group IV: (HD + VD) (Manjari et al., 2022). RESULTS: On administration of 500IU/kg/day of VD, HD mice showed a significant reduction in the gene expression of the immune receptor, TCR-beta subunit, nuclear factor kappa B (NF-kappaB), inflammatory cytokines, and key antioxidants, followed by a decrease in the acetylcholinesterase activity. CONCLUSION: A novel neuroprotective effect of VD in HD is demonstrated by combating the immune receptor, TCR-beta gene expression, antioxidant markers, and inflammatory cytokines. In addition, HD mice on VD administration for 0-15 days showed an enhancement in cholinergic signaling with restoration in alpha7 nAChRs mRNA and protein expression in the striatum and cortex.

PubMedSearch : Manjari_2023_IBRO.Neurosci.Rep_15_116
PubMedID: 38204575

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Citations formats

Manjari S, Abraham SM, Poornima R, Chaturvedi RK, Maity S, Komal P (2023)
Unprecedented effect of vitamin D3 on T-cell receptor beta subunit and alpha7 nicotinic acetylcholine receptor expression in a 3-nitropropionic acid induced mouse model of Huntington's disease
IBRO Neurosci Rep 15 :116

Manjari S, Abraham SM, Poornima R, Chaturvedi RK, Maity S, Komal P (2023)
IBRO Neurosci Rep 15 :116