| Title : Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems - Marco-Ramell_2012_J.Proteomics_75_4399 |
| Author(s) : Marco-Ramell A , Arroyo L , Saco Y , Garcia-Heredia A , Camps J , Fina M , Piedrafita J , Bassols A |
| Ref : J Proteomics , 75 :4399 , 2012 |
| Abstract : Three groups of cows representing three ranges of welfare in the production system were included in the study: two groups of Bruna dels Pirineus beef cattle maintained under different management systems (good and semiferal conditions) and a group of Alberes cows, a breed that lives in the mountains (hardest conditions). In order to identify new stress/welfare biomarkers, serum from Bruna cows living in both environments was subjected to DIGE labelling, two-dimensional electrophoresis and MALDI-MS or ion trap MS. Identification was achieved for 15 proteins, which mainly belonged to three biological functions, the oxidative stress pathway (glutathione peroxidase (GPx) and paraoxonase (PON-1)), the acute phase protein family (Heremans Schmid glycoprotein alpha2 (alpha2-HSG)) and the complement system. Biological validation included the Alberes breed. GPx and PON-1 were validated by an enzymatic assay and found to be higher and lower, respectively, in cows living in hard conditions. alpha2-HSG was validated by ELISA and found to be reduced in hard conditions. Other biomarkers of the redox status were also altered by living conditions: protein carbonyl content, superoxide dismutase (SOD) and glutathione reductase (GR). Our results show that changes in the redox system are the main adaptation of cows living in challenging environmental conditions. |
| ESTHER : Marco-Ramell_2012_J.Proteomics_75_4399 |
| PubMedSearch : Marco-Ramell_2012_J.Proteomics_75_4399 |
| PubMedID: 22543282 |
Marco-Ramell A, Arroyo L, Saco Y, Garcia-Heredia A, Camps J, Fina M, Piedrafita J, Bassols A (2012)
Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems
J Proteomics
75 :4399
Marco-Ramell A, Arroyo L, Saco Y, Garcia-Heredia A, Camps J, Fina M, Piedrafita J, Bassols A (2012)
J Proteomics
75 :4399