Title : Subpicogram determination of oxytocin by an enzyme immunoassay using acetylcholinesterase as label - Marnet_1994_J.Immunoassay_15_35 |
Author(s) : Marnet PG , Volland H , Pradelles P , Grassi J , Beaufils M |
Ref : Journal of Immunoassay , 15 :35 , 1994 |
Abstract :
The pure tetrameric form of Acetylcholinesterase (EC-3.1.1.7) from the electric eel electrophorus electricus has been covalently coupled to oxytocin. This conjugate has been used as tracer in a heterologous competitive immunoassay. Microtiter plates coated with a mouse monoclonal anti-rabbit immunoglobulin antibody were used to separate bound and free moieties of the tracer. Acetylcholinesterase activity bound to the solid phase was measured by a colorimetric assay. The minimum detectable concentration was 0.075 pg/well (ie 1.5 pg/ml) and precision was less than 8% at concentration above 0.15 pg/well. An extraction step improved sensitivity up to 10 times with good recoveries. To assess the validity of this assay, basal levels of oxytocin were measured during the oestrous cycle of a cow. |
PubMedSearch : Marnet_1994_J.Immunoassay_15_35 |
PubMedID: 8150985 |
Marnet PG, Volland H, Pradelles P, Grassi J, Beaufils M (1994)
Subpicogram determination of oxytocin by an enzyme immunoassay using acetylcholinesterase as label
Journal of Immunoassay
15 :35
Marnet PG, Volland H, Pradelles P, Grassi J, Beaufils M (1994)
Journal of Immunoassay
15 :35