| Title : A novel tannase from the xerophilic fungus Aspergillus niger GH1 - Mata-Gomez_2009_J.Microbiol.Biotechnol_19_987 |
| Author(s) : Mata-Gomez M , Rodriguez LV , Ramos EL , Renovato J , Cruz-Hernandez MA , Rodriguez R , Contreras J , Aguilar CN |
| Ref : J Microbiol Biotechnol , 19 :987 , 2009 |
| Abstract : Aspergillus niger GH1 previously isolated and identified by our group as a wild tannase producer was grown under solid-state (SSC) and submerged culture (SmC) conditions to select the enzyme production system. For tannase purification, extracellular tannase was produced under SSC using polyurethane foam as the inert support. Tannase was purified to apparent homogeneity by ultrafiltration, anion-exchange chromatography, and gel filtration that led to a purified enzyme with a specific activity of 238.14 IU/mg protein with a final yield of 0.3% and a purification fold of 46. Three bands were found on the SDS-PAG with molecular masses of 50, 75, and 100 kDa. PI of 3.5 and 7.1% Nglycosylation were noted. Temperature and pH optima were 60 degrees and 6.0 [methyl 3,4,5-trihydroxybenzoate (MTB) as substrate], respectively. Tannase was found with a KM value of 0.41 x 10-4 M and the value of Vmax was 11.03 micromoL/min at 60 degrees for MTB. Effects of several metal salts, solvents, surfactants, and typical enzyme inhibitors on tannase activity were evaluated to establish the novelty of the enzyme. Finally, the tannase from A. niger GH1 was significantly inhibited by PMSF (phenylmethylsulfonyl fluoride), and therefore, it is possible to consider the presence of a serine or cysteine residue in the catalytic site. |
| ESTHER : Mata-Gomez_2009_J.Microbiol.Biotechnol_19_987 |
| PubMedSearch : Mata-Gomez_2009_J.Microbiol.Biotechnol_19_987 |
| PubMedID: 19809257 |
Mata-Gomez M, Rodriguez LV, Ramos EL, Renovato J, Cruz-Hernandez MA, Rodriguez R, Contreras J, Aguilar CN (2009)
A novel tannase from the xerophilic fungus Aspergillus niger GH1
J Microbiol Biotechnol
19 :987
Mata-Gomez M, Rodriguez LV, Ramos EL, Renovato J, Cruz-Hernandez MA, Rodriguez R, Contreras J, Aguilar CN (2009)
J Microbiol Biotechnol
19 :987