Matsunaga_2008_J.Biol.Chem_283_28835

Reference

Title : Mycolyltransferase-mediated glycolipid exchange in Mycobacteria - Matsunaga_2008_J.Biol.Chem_283_28835
Author(s) : Matsunaga I , Naka T , Talekar RS , McConnell MJ , Katoh K , Nakao H , Otsuka A , Behar SM , Yano I , Moody DB , Sugita M
Ref : Journal of Biological Chemistry , 283 :28835 , 2008
Abstract :

Trehalose dimycolate (TDM), also known as cord factor, is a major surface glycolipid of the cell wall of mycobacteria. Because of its potent biological functions in models of infection, adjuvancy, and immunotherapy, it is important to determine how its biosynthesis is regulated. Here we show that glucose, a host-derived product that is not readily available in the environment, causes Mycobacterium avium to down-regulate TDM expression while up-regulating production of another major glycolipid with immunological roles in T cell activation, glucose monomycolate (GMM). In vitro, the mechanism of reciprocal regulation of TDM and GMM involves competitive substrate selection by antigen 85A. The switch from TDM to GMM biosynthesis occurs near the physiological concentration of glucose present in mammalian hosts. We further demonstrate that GMM is produced in vivo by mycobacteria growing in mouse lung. These results establish an enzymatic pathway for GMM production. More generally, these observations provide a specific enzymatic mechanism for dynamic alterations of cell wall glycolipid remodeling in response to the transition from noncellular to cellular growth environments, including factors that are monitored by the host immune system.

PubMedSearch : Matsunaga_2008_J.Biol.Chem_283_28835
PubMedID: 18703502
Gene_locus related to this paper: myca1-a0q9c0

Related information

Gene_locus myca1-a0q9c0

Citations formats

Matsunaga I, Naka T, Talekar RS, McConnell MJ, Katoh K, Nakao H, Otsuka A, Behar SM, Yano I, Moody DB, Sugita M (2008)
Mycolyltransferase-mediated glycolipid exchange in Mycobacteria
Journal of Biological Chemistry 283 :28835

Matsunaga I, Naka T, Talekar RS, McConnell MJ, Katoh K, Nakao H, Otsuka A, Behar SM, Yano I, Moody DB, Sugita M (2008)
Journal of Biological Chemistry 283 :28835