| Title : The immunogenicity of the acetylcholinesterases of the cattle lungworm Dictyocaulus viviparus - McKeand_1994_Internat.J.Parasitol_24_501 |
| Author(s) : McKeand JB , Knox DP , Duncan JL , Kennedy MW |
| Ref : International Journal for Parasitology , 24 :501 , 1994 |
| Abstract : Somatic extracts and excretory/secretory (ES) products of the adult stage of the cattle nematode, Dictyocaulus viviparus, were examined for acetylcholinesterase (AChE) activity. Both were found to contain activity which had an optimum pH of 9.5, however, the adult ES products contained over 200 times more AChE activity per unit protein. Gel electrophoresis and specific enzyme staining revealed 5 migratory isoforms of AChE which were common to adult ES products and adult homogenates. Comparison of L3 with L4 and adult extracts indicated that the AChE were only produced by later developmental stages of this parasite. The antigenicity of D. viviparus AChE was demonstrated by binding to serum IgG from naturally and experimentally infected calves but the enzymes were not recognized by calves vaccinated twice with 400 Gy-irradiated larvae. This is the first report of helminth AChE release by a parasitic nematode in a pulmonary location. The presence of these enzymes in such high amounts in the ES products, along with their immunogenicity, suggests that they might have an important role to play in the immunobiology of D. viviparus in the lungs. |
| ESTHER : McKeand_1994_Internat.J.Parasitol_24_501 |
| PubMedSearch : McKeand_1994_Internat.J.Parasitol_24_501 |
| PubMedID: 8082980 |
McKeand JB, Knox DP, Duncan JL, Kennedy MW (1994)
The immunogenicity of the acetylcholinesterases of the cattle lungworm Dictyocaulus viviparus
International Journal for Parasitology
24 :501
McKeand JB, Knox DP, Duncan JL, Kennedy MW (1994)
International Journal for Parasitology
24 :501