Melnikova_2000_Eur.J.Pharmacol_393_75

Reference

Title : Interactions between regulatory proteins that bind to the nicotinic receptor beta4 subunit gene promoter - Melnikova_2000_Eur.J.Pharmacol_393_75
Author(s) : Melnikova IN , Yang Y , Gardner PD
Ref : European Journal of Pharmacology , 393 :75 , 2000
Abstract :

The genes encoding the alpha3, alpha5 and beta4 subunits of nicotinic acetylcholine receptors are tightly clustered within the genome. As these three subunits constitute the predominant acetylcholine receptor subtype expressed in the peripheral nervous system, their genomic proximity suggests a regulatory mechanism ensuring their coordinate expression. We previously identified two transcriptional regulatory elements within the beta4 promoter. One of these elements, a CT box, interacts with the regulatory factors heterogeneous nuclear ribonucleoprotein K and Puralpha. Another element, a CA box, interacts with Sp1 and Sp3. The binding site for a fifth factor, Sox10, overlaps the CT and CA boxes. As the CT and CA boxes are adjacent, we postulated that the proteins that bind to the elements interact. Here we report that the CT box-binding factors interact with each other as do the CA box-binding factors. However, there are no direct associations between the two pairs of proteins. Interestingly though, Sox10 directly interacts with all four proteins, suggesting a central role in beta4 gene expression for this member of the Sox family of regulatory factors.

PubMedSearch : Melnikova_2000_Eur.J.Pharmacol_393_75
PubMedID: 10771000

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Citations formats

Melnikova IN, Yang Y, Gardner PD (2000)
Interactions between regulatory proteins that bind to the nicotinic receptor beta4 subunit gene promoter
European Journal of Pharmacology 393 :75

Melnikova IN, Yang Y, Gardner PD (2000)
European Journal of Pharmacology 393 :75