| Title : Identification of a new thyroglobulin variant: a guanine-to-adenine transition resulting in the substitution of arginine 2510 by glutamine - Mendive_1997_Thyroid_7_587 |
| Author(s) : Mendive FM , Rossetti LC , Vassart G , Targovnik HM |
| Ref : Thyroid , 7 :587 , 1997 |
|
Abstract :
We analyzed thyroglobulin (Tg) reverse transcription polymerase chain reaction (RT-PCR) products from three congenital goiters and three normal thyroid tissues by Taq I digestion. Tg coding sequences were amplified from position 57 to 8448 in 12 amplification fragments. A Taq I restriction fragment length polymorphism was detected in the most 3' RT-PCR product (nt 7584 through 8448). Data from the sequence showed a G-->A transition (nt 7627) causing the disappearance of the Taq I site in position 7625. It produced the substitution of arginine for a glutamine at position 2510. Afterwards, we established that the glutamine allele is present in normal unrelated individuals, with an allelic frequency of 62%. This Tg variant is thus widely represented in the human population. The available sequence information from rat and bovine Tg showed the presence, in both, of glutamine at position 2510. |
| PubMedSearch : Mendive_1997_Thyroid_7_587 |
| PubMedID: 9292947 |
Mendive FM, Rossetti LC, Vassart G, Targovnik HM (1997)
Identification of a new thyroglobulin variant: a guanine-to-adenine transition resulting in the substitution of arginine 2510 by glutamine
Thyroid
7 :587
Mendive FM, Rossetti LC, Vassart G, Targovnik HM (1997)
Thyroid
7 :587