Title : Acetylcholine receptor degradation in adult rat diaphragms in organ culture and the effect of anti-acetylcholine receptor antibodies - Merlie_1979_J.Biol.Chem_254_6320 |
Author(s) : Merlie JP , Heinemann S , Lindstrom JM |
Ref : Journal of Biological Chemistry , 254 :6320 , 1979 |
Abstract :
Acetylcholine receptor located at the neuromuscular synapse of normal innervated adult muscle fibers is extremely stable metabolically. We have studied the kinetics of receptor degradation in both normal innervated and denervated rat diaphragms in organ culture. These studies show that degradation of receptor-bound 125I-alpha-bungarotoxin is a valid measure of junctional receptor degradation. Degradation of junctional receptor is similar or identical to degradation of extrajunctional receptor in many ways: 1) both require energy, 2) both are inhibited by specific lysosomal protease inhibitors, 3) both are inhibited by treatment with colchicine, and 4) both are stimulated by treatment with anti-acetylcholine receptor antibodies. The one important distinction between degradation of junctional and extrajunctional receptor is a 10-fold difference in rate constant for the process. |
PubMedSearch : Merlie_1979_J.Biol.Chem_254_6320 |
PubMedID: 447716 |
Merlie JP, Heinemann S, Lindstrom JM (1979)
Acetylcholine receptor degradation in adult rat diaphragms in organ culture and the effect of anti-acetylcholine receptor antibodies
Journal of Biological Chemistry
254 :6320
Merlie JP, Heinemann S, Lindstrom JM (1979)
Journal of Biological Chemistry
254 :6320