| Title : Role of sphingosine kinase in Ca(2+) signalling by epidermal growth factor receptor - Meyer_1999_FEBS.Lett_461_217 |
| Author(s) : Meyer zu Heringdorf D , Lass H , Kuchar I , Alemany R , Guo Y , Schmidt M , Jakobs KH |
| Ref : FEBS Letters , 461 :217 , 1999 |
|
Abstract :
Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca(2+) signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca(2+) by intracellular SPP. In HEK3 cells, EGF increased [Ca(2+)](i) and stimulated both, SPK and PLC. [Ca(2+)](i) increase, but not PLC stimulation, was strongly reduced by SPK inhibition. In HEK2 cells, EGF similarly stimulated PLC, but did not increase [Ca(2+)](i) or stimulate SPK, suggesting that intracellular SPP production plays a major role for Ca(2+) signalling by EGF in HEK-293 cells. |
| PubMedSearch : Meyer_1999_FEBS.Lett_461_217 |
| PubMedID: 10567700 |
Meyer zu Heringdorf D, Lass H, Kuchar I, Alemany R, Guo Y, Schmidt M, Jakobs KH (1999)
Role of sphingosine kinase in Ca(2+) signalling by epidermal growth factor receptor
FEBS Letters
461 :217
Meyer zu Heringdorf D, Lass H, Kuchar I, Alemany R, Guo Y, Schmidt M, Jakobs KH (1999)
FEBS Letters
461 :217