Miao_2006_J.Ind.Microbiol.Biotechnol_33_129

Reference

Title : The lipopeptide antibiotic A54145 biosynthetic gene cluster from Streptomyces fradiae - Miao_2006_J.Ind.Microbiol.Biotechnol_33_129
Author(s) : Miao V , Brost R , Chapple J , She K , Gal MF , Baltz RH
Ref : J Ind Microbiol Biotechnol , 33 :129 , 2006
Abstract :

Ca(2+)-dependent cyclic lipodepsipeptides are an emerging class of antibiotics for the treatment of infections caused by Gram-positive pathogens. These compounds are synthesized by nonribosomal peptide synthetase (NRPS) complexes encoded by large gene clusters. The gene cluster encoding biosynthetic pathway enzymes for the Streptomyces fradiae A54145 NRP was cloned from a cosmid library and characterized. Four NRPS-encoding genes, responsible for subunits of the synthetase, as well as genes for accessory functions such as acylation, methylation and hydroxylation, were identified by sequence analysis in a 127 kb region of DNA that appears to be located subterminally in the bacterial chromosome. Deduced epimerase domain-encoding sequences within the NRPS genes indicated a D: -stereochemistry for Glu, Lys and Asn residues, as observed for positionally analogous residues in two related compounds, daptomycin, and the calcium-dependent antibiotic (CDA) produced by Streptomyces roseosporus and Streptomyces coelicolor, respectively. A comparison of the structure and the biosynthetic gene cluster of A54145 with those of the related peptides showed many similarities. This information may contribute to the design of experiments to address both fundamental and applied questions in lipopeptide biosynthesis, engineering and drug development.

PubMedSearch : Miao_2006_J.Ind.Microbiol.Biotechnol_33_129
PubMedID: 16208464
Gene_locus related to this paper: strfr-q45r80 , strfr-q45r82 , strfr-q45ra2 , strfr-q45rb2

Related information

Gene_locus strfr-q45r80    strfr-q45r82    strfr-q45ra2    strfr-q45rb2
Gene_locus_frgt strfr-q45ra5

Citations formats

Miao V, Brost R, Chapple J, She K, Gal MF, Baltz RH (2006)
The lipopeptide antibiotic A54145 biosynthetic gene cluster from Streptomyces fradiae
J Ind Microbiol Biotechnol 33 :129

Miao V, Brost R, Chapple J, She K, Gal MF, Baltz RH (2006)
J Ind Microbiol Biotechnol 33 :129