| Title : Enhanced enzyme activity and enantioselectivity of lipases in organic solvents by crown ethers and cyclodextrins - Mine_2003_J.Biosci.Bioeng_95_441 |
| Author(s) : Mine Y , Fukunaga K , Itoh K , Yoshimoto M , Nakao K , Sugimura Y |
| Ref : J Biosci Bioeng , 95 :441 , 2003 |
| Abstract : Lipases from Candida rugosa (CRL) and Pseudomonas cepacia (PCL) were co-lyophilized with cyclic oligoethers including four crown ethers and nine cyclodextrins (CyDs), and their transesterification activity and enantioselectivity in organic solvents were evaluated. The PCL co-lyophilized with each additive showed simultaneously enhanced enzyme activity and enantioselectivity when compared to the native lipase lyophilized from buffer alone; in contrast, such enhancement was not observed for the co-lyophilized CRL. Among the cyclic oligoethers examined, permethylated betaCyD (Me1.78betaCyD), as the most suitable additive, was used for the optimization of both the co-lyophilized PCL preparation and reaction conditions by determining the effects of varying the additive/lipase ratio, aqueous pH, the nature of organic solvents, and temperature. The initial rate determined for the transesterification between racemic 2,2-dimethyl-1,3-dioxolane-4-methanol and vinyl butyrate in diisopropyl ether at 30 degrees C increased by up to 17-fold and the enantioselectivity represented by E could be doubled. While there was an inverse correlation between temperature and enantioselectivity, with the Me1.78betaCyD-PCL co-lyophilizate, the reaction rate even at 0 degrees C was much higher than that at higher temperatures in the native PCL-catalyzed reaction. Hence, this method seems to be of practical use for the large-scale production of optically active compounds. |
| ESTHER : Mine_2003_J.Biosci.Bioeng_95_441 |
| PubMedSearch : Mine_2003_J.Biosci.Bioeng_95_441 |
| PubMedID: 16233437 |
Mine Y, Fukunaga K, Itoh K, Yoshimoto M, Nakao K, Sugimura Y (2003)
Enhanced enzyme activity and enantioselectivity of lipases in organic solvents by crown ethers and cyclodextrins
J Biosci Bioeng
95 :441
Mine Y, Fukunaga K, Itoh K, Yoshimoto M, Nakao K, Sugimura Y (2003)
J Biosci Bioeng
95 :441