Minggu_2023_MethodsX_11_102434

Reference

Title : Seamless and orthogonal expression of genetic parts in polyhydroxyalkanoate (PHA)-producing bacterial chassis for plastic bio-upcycling applications - Minggu_2023_MethodsX_11_102434
Author(s) : Minggu MM , Naseron NAH , Shaberi HSA , Muhammad NAN , Baharum SN , Ramzi AB
Ref : MethodsX , 11 :102434 , 2023
Abstract :

Polyhydroxyalkanoate (PHA)-producing bacteria represent a powerful synthetic biology chassis for waste bioconversion and bio-upcycling where PHAs can be produced as the final products. In this study, we present a seamless plasmid construction for orthogonal expression of recombinant PET hydrolase (PETase) in model PHA-producing bacteria P. putida and C. necator. To this end, this study described seamless cloning and expression methods utilizing SureVector (SV) system for generating pSV-Ortho-PHA (pSVOP) expression platform in bioengineered P. putida and C. necator. Genetic parts specifically Trc promoter, pBBR1 origin of replication, anchoring proteins and signal sequences were utilized for the transformation of pSVOP-based plasmid in electrocompetent cells and orthogonal expression of PETase in both P. putida and C. necator. Validation steps in confirming functional expression of PETase activity in corresponding PETase-expressing strains were also described to demonstrate seamless and detailed methods in establishing bioengineered P. putida and C. necator as whole-cell biocatalysts tailored for plastic bio-upcycling.Seamless plasmid construction for orthogonal expression in PHA-producing bacteria.Step-by-step guide for high-efficiency generation of electrotransformants of P. putida and C. necator.Adaptable methods for rapid strain development (Design, Build, Test and Learn) for whole-cell biocatalysis.

PubMedSearch : Minggu_2023_MethodsX_11_102434
PubMedID: 37846354

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Citations formats

Minggu MM, Naseron NAH, Shaberi HSA, Muhammad NAN, Baharum SN, Ramzi AB (2023)
Seamless and orthogonal expression of genetic parts in polyhydroxyalkanoate (PHA)-producing bacterial chassis for plastic bio-upcycling applications
MethodsX 11 :102434

Minggu MM, Naseron NAH, Shaberi HSA, Muhammad NAN, Baharum SN, Ramzi AB (2023)
MethodsX 11 :102434