| Title : Chemical synthesis of N-acetylcysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography\/electrospray ionization-linear ion trap mass spectrometry - Mitamura_2009_J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci_877_2630 |
| Author(s) : Mitamura K , Watanabe S , Sakai T , Okihara R , Sogabe M , Wakamiya T , Hofmann AF , Ikegawa S |
| Ref : Journal of Chromatography B Analyt Technol Biomed Life Sciences , 877 :2630 , 2009 |
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Abstract :
N-Acetylcysteine (NAC) conjugates of the five major bile acids occurring in man were synthesized in order to investigate the possible formation in vivo of these conjugates. Upon collision-induced dissociation, structurally informative daughter ions were observed. The transformation of cholyl-adenylate and cholyl-CoA thioester into a N-acetyl-S-(cholyl)cysteine by rat hepatic glutathione S-transferase was confirmed by liquid chromatography/electrospray ionization-linear ion trap mass spectrometry (LC/ESI-MS(2)). Lithocholic acid was administered orally to bile duct-ligated rats that also received NAC intraperitoneally. The NAC conjugate of lithocholic acid was identified in urine by means of LC/ESI-MS(2). Rapid hydrolysis of the BA-NAC conjugates by rabbit liver carboxylesterase was found, demonstrating the possible labile nature of the NAC conjugates formed in the liver. |
| PubMedSearch : Mitamura_2009_J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci_877_2630 |
| PubMedID: 19346170 |
Mitamura K, Watanabe S, Sakai T, Okihara R, Sogabe M, Wakamiya T, Hofmann AF, Ikegawa S (2009)
Chemical synthesis of N-acetylcysteine conjugates of bile acids and in vivo formation in cholestatic rats as shown by liquid chromatography\/electrospray ionization-linear ion trap mass spectrometry
Journal of Chromatography B Analyt Technol Biomed Life Sciences
877 :2630
Mitamura K, Watanabe S, Sakai T, Okihara R, Sogabe M, Wakamiya T, Hofmann AF, Ikegawa S (2009)
Journal of Chromatography B Analyt Technol Biomed Life Sciences
877 :2630