Mogana_2013_Evid.Based.Complement.Alternat.Med_2013_734824

Bioassay guided fractionation of an ethanol extract of leaves of Canarium patentinervium Miq. (Burseraceae Kunth.) led to the isolation of scopoletin. The structure of this coumarin was elucidated based on spectroscopic methods including nuclear magnetic resonance (NMR-1D and 2D) and mass spectrometry. Scopoletin inhibited the enzymatic activity of 5-lipoxygenase and acetyl cholinesterase with an IC50 equal to 1.76 +/- 0.01 mu M and 0.27 +/- 0.02 mM, respectively, and confronted oxidation in the ABTS, DPPH, FRAP, and beta -carotene bleaching assay with EC50 values equal to 5.62 +/- 0.03 mu M, 0.19 +/- 0.01 mM, 0.25 +/- 0.03 mM and 0.65 +/- 0.07 mM, respectively. Given the aforementioned evidence, it is tempting to speculate that scopoletin represents an exciting scaffold from which to develop leads for treatment of neurodegenerative diseases.