Title : Colocalization of cholinesterases with beta amyloid protein in aged and Alzheimer's brains - Moran_1993_Acta.Neuropath_85_362 |
Author(s) : Moran MA , Mufson EJ , Gomez-Ramos P |
Ref : Acta Neuropathologica , 85 :362 , 1993 |
Abstract :
The colocalization of beta amyloid protein with the enzymes acetyl- and butyrylcholinesterase was assessed using immunocytochemistry for beta amyloid protein and a sensitive histochemical technique for cholinesterases. In non-demented aged and Alzheimer's disease brains, double-stained sections for cholinesterases and thioflavin-S showed that all thioflavin-S-positive plaques were also positive for cholinesterases, indicating the presence of these enzymes in all plaques with beta-pleated amyloid protein. When amyloid angiopathy was present, cholinesterases were also observed in amyloid-laden vessels walls. Comparison of series of adjacent sections alternatively stained for acetylcholinesterase, beta amyloid protein and butyrylcholinesterase, as well as by double histo-immunocytochemical staining, showed either cholinesterase in a proportion of the preamyloid diffuse plaques. These data indicate that cholinesterases are associated with the amyloid protein from very early stages, when the beta-pleated structure is being formed. Novel functions attributed to acetyl- and butyrylcholinesterase, such us their proteolytic activity either by themselves or in association with heparan sulfate proteoglycans, may play a role in the aggregation or the consolidation processes taking place at the early stages of diffuse plaque formation. |
PubMedSearch : Moran_1993_Acta.Neuropath_85_362 |
PubMedID: 8480510 |
Moran MA, Mufson EJ, Gomez-Ramos P (1993)
Colocalization of cholinesterases with beta amyloid protein in aged and Alzheimer's brains
Acta Neuropathologica
85 :362
Moran MA, Mufson EJ, Gomez-Ramos P (1993)
Acta Neuropathologica
85 :362