Moreno_2014_BMC.Genomics_15_26

Reference

Title : Genomic comparison of sporeforming bacilli isolated from milk - Moreno_2014_BMC.Genomics_15_26
Author(s) : Moreno Switt AI , Andrus AD , Ranieri ML , Orsi RH , Ivy R , den Bakker HC , Martin NH , Wiedmann M , Boor KJ
Ref : BMC Genomics , 15 :26 , 2014
Abstract :

BACKGROUND: Sporeformers in the order Bacillales are important contributors to spoilage of pasteurized milk. While only a few Bacillus and Viridibacillus strains can grow in milk at 6 degrees C, the majority of Paenibacillus isolated from pasteurized fluid milk can grow under these conditions. To gain a better understanding of genomic features of these important spoilage organisms and to identify candidate genomic features that may facilitate cold growth in milk, we performed a comparative genomic analysis of selected dairy associated sporeformers representing isolates that can and cannot grow in milk at 6 degrees C.
RESULTS: The genomes for seven Paenibacillus spp., two Bacillus spp., and one Viridibacillus sp. isolates were sequenced. Across the genomes sequenced, we identified numerous genes encoding antimicrobial resistance mechanisms, bacteriocins, and pathways for synthesis of non-ribosomal peptide antibiotics. Phylogenetic analysis placed genomes representing Bacillus, Paenibacillus and Viridibacillus into three distinct well supported clades and further classified the Paenibacillus strains characterized here into three distinct clades, including (i) clade I, which contains one strain able to grow at 6 degrees C in skim milk broth and one strain not able to grow under these conditions, (ii) clade II, which contains three strains able to grow at 6 degrees C in skim milk broth, and (iii) clade III, which contains two strains unable to grow under these conditions. While all Paenibacillus genomes were found to include multiple copies of genes encoding beta-galactosidases, clade II strains showed significantly higher numbers of genes encoding these enzymes as compared to clade III strains. Genome comparison of strains able to grow at 6 degrees C and strains unable to grow at this temperature identified numerous genes encoding features that might facilitate the growth of Paenibacillus in milk at 6 degrees C, including peptidases with cold-adapted features (flexibility and disorder regions in the protein structure) and cold-adaptation related proteins (DEAD-box helicases, chaperone DnaJ).
CONCLUSIONS: Through a comparative genomics approach we identified a number of genomic features that may relate to the ability of selected Paenibacillus strains to cause spoilage of refrigerated fluid milk. With additional experimental evidence, these data will facilitate identification of targets to detect and control Gram positive spore formers in fluid milk.

PubMedSearch : Moreno_2014_BMC.Genomics_15_26
PubMedID: 24422886
Gene_locus related to this paper: bacce-c2zyv1 , bacce-BC2337 , bacce-BC5130 , geos4-d3ed06 , geos4-d3edx2 , 9bacl-w4bas9 , 9bacl-w4drs0 , 9bacl-w4bws1 , 9bacl-w4b6j5 , 9bacl-w4f050 , 9bacl-w4c3j8 , 9bacl-w4b844 , 9bacl-w4cb60 , 9bacl-w4bk58 , 9bacl-w4dle8 , 9bacl-w4avh4 , 9bacl-w4bpv3 , 9bacl-w4cbt0 , 9bacl-w4eid3 , 9bacl-w4en52 , 9bacl-w4els5 , 9bacl-w4ej46 , 9bacl-w4eqh1 , 9bacl-a0a089mve8 , 9bacl-w4e0c1 , 9bacl-w4ah68 , 9bacl-w4ccg0 , 9bacl-w4eha7 , 9bacl-w4ag71

Related information

Gene_locus bacce-c2zyv1    bacce-BC2337    bacce-BC5130    geos4-d3ed06    geos4-d3edx2    9bacl-w4bas9    9bacl-w4drs0    9bacl-w4bws1    9bacl-w4b6j5    9bacl-w4f050    9bacl-w4c3j8    9bacl-w4b844    9bacl-w4cb60    9bacl-w4bk58    9bacl-w4dle8    9bacl-w4avh4    9bacl-w4bpv3    9bacl-w4cbt0    9bacl-w4eid3    9bacl-w4en52    9bacl-w4els5    9bacl-w4ej46    9bacl-w4eqh1    9bacl-a0a089mve8    9bacl-w4e0c1    9bacl-w4ah68    9bacl-w4ccg0    9bacl-w4eha7    9bacl-w4ag71

Citations formats

Moreno Switt AI, Andrus AD, Ranieri ML, Orsi RH, Ivy R, den Bakker HC, Martin NH, Wiedmann M, Boor KJ (2014)
Genomic comparison of sporeforming bacilli isolated from milk
BMC Genomics 15 :26

Moreno Switt AI, Andrus AD, Ranieri ML, Orsi RH, Ivy R, den Bakker HC, Martin NH, Wiedmann M, Boor KJ (2014)
BMC Genomics 15 :26