Morley_1998_Brain.Res.Mol.Brain.Res_53_78

Reference

Title : Identification of the subunits of the nicotinic cholinergic receptors in the rat cochlea using RT-PCR and in situ hybridization - Morley_1998_Brain.Res.Mol.Brain.Res_53_78
Author(s) : Morley BJ , Li HS , Hiel H , Drescher DG , Elgoyhen AB
Ref : Brain Research Mol Brain Res , 53 :78 , 1998
Abstract :

There are two tissues in the adult mammalian cochlea that are post-synaptic to cholinergic efferent fibers: The outer hair cells (OHCs) and the dendrites of the afferent fibers of the type I spiral ganglion cells. The unusual nicotinic-like pharmacology of cochlear cholinergic responses and the unique embryonic development of cochlear tissues suggest that the inner-ear nicotinic cholinergic receptor (nAChR) may be different from nAChRs described previously at synapses in the mammalian brain, autonomic ganglia, or skeletal muscle. In this study, we determined the mRNA expression of the alpha2-7, alpha9, and beta2-4 subunits of the nicotinic acetylcholine receptor (nAChR) family in the rat cochlea. In micro-dissected tissue from the organ of Corti, spiral ganglion, and the membranous lateral wall, we found mRNA expression of the alpha7 and alpha9 subunits in the organ of Corti and alpha5-7, and beta2 and beta3 in the spiral ganglion using RT-PCR. Employing in situ hybridization with 35S-riboprobes, we localized alpha9 in hair cells regions and alpha6, alpha7 and beta2 in the type I cells of the spiral ganglion. No evidence of nAChR subunit mRNA expression was found in supporting cells, but beta2 was expressed in type II spiral ganglion cells, which are neither cholinergic nor cholinoceptive.

PubMedSearch : Morley_1998_Brain.Res.Mol.Brain.Res_53_78
PubMedID: 9473597

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Citations formats

Morley BJ, Li HS, Hiel H, Drescher DG, Elgoyhen AB (1998)
Identification of the subunits of the nicotinic cholinergic receptors in the rat cochlea using RT-PCR and in situ hybridization
Brain Research Mol Brain Res 53 :78

Morley BJ, Li HS, Hiel H, Drescher DG, Elgoyhen AB (1998)
Brain Research Mol Brain Res 53 :78