Mussadiq_2013_J.Asian.Nat.Prod.Res_15_708

Chromatographic purification of ethyl acetate soluble fraction of the methanolic extract of the flowers of Aerva javanica yielded three new acylated flavone glycosides: kaempferol-3-O-beta-d-[4'''-E-p-coumaroyl-alpha-l-rhamnosyl(1 --> 6)]-galactoside (1), kaempferol-3-O-beta-d-[4'''-E-p-coumaroyl-alpha-l-rhamnosyl(1 --> 6)]-(3''-E-p-coumaroyl)galactoside (2), and kaempferol-3-O-beta-d-[4'''-E-p-coumaroyl-alpha-l-rhamnosyl(1 --> 6)]-(4''-E-p-coumaroyl)galactoside (3), along with p-coumaric acid (4), caffeic acid (5), gallic acid (6), eicosanyl-trans-p-coumarate (7), hexadecyl ferulate (8), and hexacosyl ferulate (9). The compounds 1-9 were characterized using 1D ((1)H, (13)C) and 2D NMR (HMQC, HMBC, and COSY) spectroscopy and mass spectrometry (EI-MS, HR-EI-MS, FAB-MS, and HR-FAB-MS) and in comparison with the reported data in the literature. Compound 1 showed weak inhibitory activity against enzymes, such as acetylcholinesterase, butyrylcholinesterase, and lipoxygenase with IC50 values 205.1, 304.1, and 212.3 muM, respectively, whereas compounds 2 and 3 were only weakly active against the enzyme acetylcholinesterase.