Mussini_1984_Neurosci_12_53

Degeneration of neuromuscular junctions in the iris muscle was observed to have occurred 4 days after ciliary nerve crush. By day 10 reinnervation had commenced and by day 30 the maturation of neuromuscular junctions was nearly complete. The loss and recovery of acetylcholinesterase activity in the iris muscle paralleled the denervation-reinnervation process, with recovery being completed by day 30, whereas the loss in the activity of choline acetyltransferase had not yet completely recovered at this time. The acetylcholinesterase activity localized cytochemically at synaptic sites followed the same trend as the total activity in the iris muscle, whereas acetylcholinesterase localized at myo-muscular junctions showed only slight changes. The acetylcholinesterase molecular forms displayed changes in their relative proportions, which could be related to the time course of the denervation-reinnervation process and to the cytochemical localization of the activity.