Mustafa_2014_Urology_84_1549 e1

OBJECTIVE: To examine the effects of neostigmine, an acetylcholinesterase inhibitor that has been used to treat impaired bladder emptying on diabetic rat urinary bladder smooth muscle.
METHODS: Rat urinary detrusor muscle strips were suspended in organ baths containing Krebs' solution for isometric tension recording. Streptozotocin-diabetic (12 weeks) bladder tissue activity compared with control was assessed by using electrical field stimulation (EFS) in the presence and absence of the cholinesterase inhibitor, neostigmine.
RESULTS: EFS-induced contractions; a major part of it is cholinergic in origin. Neostigmine significantly enhanced EFS-induced contractions in diabetic strips than control at all frequencies. Neostigmine caused concentration-dependent contractions of control and diabetic bladder tissues, which were completely abolished by atropine. Carbachol-induced bladder contraction was significantly reduced in diabetes. CONCLUSION: In diabetes mellitus, cholinesterase modulation (increase) may play a role in the development of inadequate bladder contraction, seen in later stage diabetic bladder dysfunction.