Nascimento_2016_Sci.Rep_6_18598

Reference

Title : The Type II Secreted Lipase\/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines - Nascimento_2016_Sci.Rep_6_18598
Author(s) : Nascimento R , Gouran H , Chakraborty S , Gillespie HW , Almeida-Souza HO , Tu A , Rao BJ , Feldstein PA , Bruening G , Goulart LR , Dandekar AM
Ref : Sci Rep , 6 :18598 , 2016
Abstract : Pierce's disease (PD) of grapevines is caused by Xylella fastidiosa (Xf), a xylem-limited gamma-proteobacterium that is responsible for several economically important crop diseases. The occlusion of xylem elements and interference with water transport by Xf and its associated biofilm have been posited as the main cause of PD symptom development; however, Xf virulence mechanisms have not been described. Analysis of the Xf secretome revealed a putative lipase/esterase (LesA) that was abundantly secreted in bacterial culture supernatant and was characterized as a protein ortholog of the cell wall-degrading enzyme LipA of Xanthomonas strains. LesA was secreted by Xf and associated with a biofilm filamentous network. Additional proteomic analysis revealed its abundant presence in outer membrane vesicles (OMVs). Accumulation of LesA in leaf regions associated positively with PD symptoms and inversely with bacterial titer. The lipase/esterase also elicited a hypersensitive response in grapevine. Xf lesA mutants were significantly deficient for virulence when mechanically inoculated into grapevines. We propose that Xf pathogenesis is caused by LesA secretion mediated by OMV cargos and that its release and accumulation in leaf margins leads to early stages of observed PD symptoms.
ESTHER : Nascimento_2016_Sci.Rep_6_18598
PubMedSearch : Nascimento_2016_Sci.Rep_6_18598
PubMedID: 26753904
Gene_locus related to this paper: xylfa-XF0357

Related information

Gene_locus related to this paper: xylfa-XF0357

Citations formats

Nascimento R, Gouran H, Chakraborty S, Gillespie HW, Almeida-Souza HO, Tu A, Rao BJ, Feldstein PA, Bruening G, Goulart LR, Dandekar AM (2016)
The Type II Secreted Lipase\/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines
Sci Rep 6 :18598

Nascimento R, Gouran H, Chakraborty S, Gillespie HW, Almeida-Souza HO, Tu A, Rao BJ, Feldstein PA, Bruening G, Goulart LR, Dandekar AM (2016)
Sci Rep 6 :18598