Nazir_2024_Int.J.Biol.Macromol__129345

Reference

Title : Recombinant production and characterization of a metal ion-independent Lysophospholipase from a hyperthermophilic archaeon Pyrococcus abyssi DSM25543 - Nazir_2024_Int.J.Biol.Macromol__129345
Author(s) : Nazir A , Shad M , Rashid N , Azim N , Sajjad M
Ref : Int J Biol Macromol , :129345 , 2024
Abstract :

Genome sequence of Pyrococcus abyssi DSM25543 contains a coding sequence (PAB_RS01410) for alpha/beta hydrolase (WP_010867387.1). Structural analysis revealed the presence of a consensus motif GXSXG and a highly conserved catalytic triad in the amino acid sequence of alpha/beta hydrolase that were characteristic features of lysophospholipases. A putative lysophospholipase from P. abyssi with its potential applications in oil degumming and starch processing was heterologously produced in E. coli Rosetta (DE3) pLysS in soluble form followed by its purification and characterization. The recombinant enzyme was found to be active at temperature of 40-90 degreesC and pH 5.5-7.0. However, the enzyme exhibited its optimum activity at 65 degreesC and pH 6.5. None of the metal ions (Mn(2+), Mg(2+), Ni(2+), Cu(2+), Fe(2+), Co(2+), Zn(2+) and Ca(2+)) being tested had stimulatory effect on lysophospholipase activity. K(m) and V(max) for hydrolysis of 4-nitrophenyl butyrate were calculated to be 1 +/- 0.089 mM and 1637 +/- 24.434 U/mg, respectively. It is the first report on the soluble production and characterization of recombinant lysophospholipase from P. abyssi which exhibits its lipolytic activity in the absence of divalent metal ions. Broad substrate specificity, activity and stability at elevated temperatures make recombinant lysophospholipase an ideal candidate for potential industrial applications.

PubMedSearch : Nazir_2024_Int.J.Biol.Macromol__129345
PubMedID: 38219941
Gene_locus related to this paper: pyrab-PAB1050

Related information

Gene_locus pyrab-PAB1050

Citations formats

Nazir A, Shad M, Rashid N, Azim N, Sajjad M (2024)
Recombinant production and characterization of a metal ion-independent Lysophospholipase from a hyperthermophilic archaeon Pyrococcus abyssi DSM25543
Int J Biol Macromol :129345

Nazir A, Shad M, Rashid N, Azim N, Sajjad M (2024)
Int J Biol Macromol :129345